Isolation and Purification of Rat Placental Lactogen
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چکیده
منابع مشابه
Isolation, purification, and characterization of mouse placental lactogen.
Mouse placental lactogen was purified 1840-fold from BALB/c placentae from days 14-18 of gestation with an overall yield of 29%. The purification procedure included alkaline homogenization and extraction, ammonium sulfate precipitation, hydrophobic interaction chromatography on Phenyl-Sepharose, ion-exchange chromatography on CM- and DEAE-cellulose, and gel exclusion chromatography on Sephadex ...
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Bovine placental lactogen (bPL) was purified 4,200-fold from cotyledon homogenates by 1) salt precipitation and 2) ultrafiltration, followed by 3) gel filtration, 4) anion exchange, 5) hydroxylapatite, 6) chromatofocusing, and 7) final gel filtration chromatography. Purification was monitored by radioreceptor assay (RRA) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. During chro...
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A messenger activity for HPL was identified in normal human term placentas. The mRNA was translated in rabbit reticulocyte cell-free system. The HPL synthesized was quantified by a specific immunoprecipitation and further identified by electrophoresis on sodium dodecyl sulfate polyacrylamide gel. The HPL synthesized in the reticulocyte lysate exhibited a molecular weight between 20,000 and 22,0...
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We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage lambda gt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDa polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-I...
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Progesterone, total oestrogens (mainly conjugated and unconjugated oestrone), prolactin and placental lactogen have been determined during late pregnancy in the peripheral plasma of intact or hypophysectomized ewes after destruction of the foetal pituitary. During the prolongation of gestation due to the removal of the foetal hypophysis, progesterone and placental lactogen remained at a high le...
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ژورنال
عنوان ژورنال: Nihon Chikusan Gakkaiho
سال: 1977
ISSN: 1346-907X,1880-8255
DOI: 10.2508/chikusan.48.11_667